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Recombineering homologe recombinatie in Constructen<em> Drosophila</em
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Recombineering Homologous Recombination Constructs in Drosophila
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Recombineering homologe recombinatie in Constructen<em> Drosophila</em

DOI:
10.3791/50346-v

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14:23 min

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July 13, 2013

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, , , , ,
1Department of Molecular Biology,University of Texas Southwestern Medical Center at Dallas, 2Department of Physiology,University of Texas Southwestern Medical Center at Dallas, 3Green Center for Systems Biology,University of Texas Southwestern Medical Center at Dallas

Chapters

  • 00:05Title
  • 01:53Selection of BAC Clone and Region to Target
  • 03:04Insert the Homology Arms into P[acman]-KO 1.0
  • 04:40Recombining the Genomic Region of Interest into P[acman]-KO 1.0
  • 08:59Replacing the Genomic Region with the Targeting Cassette
  • 12:30Anticipated Yields
  • 13:42Conclusion

Summary

Automatic Translation

Automatic Translation

Homologe recombinatie technieken sterk vooruit<em> Drosophila</em> Genetica doordat de vorming van moleculair precieze mutaties. De recente goedkeuring van recombineering maakt het mogelijk om grote stukken DNA te manipuleren en transformeren ze in<em> Drosophila</em<sup> 6</sup>. De methoden die hier gepresenteerde combineren van deze technieken om snel te genereren grote vectoren voor homologe recombinatie.

Tags

Keywords: RecombineeringHomologous RecombinationDrosophilaBAC TransgenesisEnds-out Homologous RecombinationGateway TechnologyGene TargetingKnockoutKnock-inGenomic Manipulation

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