Journal
/
Biology
/
核小体阵列从重组核心组蛋白和核小体定位的DNA组装
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA
Please note that all translations are automatically generated. Click here for the English version.

核小体阵列从重组核心组蛋白和核小体定位的DNA组装

DOI:
10.3791/50354-v

•

10:40 min

•

September 10, 2013

•

, , , , ,
1Biochemistry and Molecular Biology,Colorado State University

Chapters

  • 00:05Title
  • 01:37Assembly of Recombinant Core Histones into Octamers
  • 03:14Purification of Histone Octamers by Size Exclusion Chromatography |
  • 05:32Sedimentation Velocity Analysis of Reconstituted Nucleosomal Arrays
  • 07:50Results: Nucleosomal Arrays from Recombinant Xenopus Core Histones
  • 09:56Conclusion

Summary

Automatic Translation

Automatic Translation

的方法，提出了用于从重组核心组蛋白模型核小体阵列的重构和串联重复的核小体定位的DNA。我们还描述沉降速度实验在分析型超速离心机，和原子力显微镜（AFM）是如何被用来监测核小体阵列饱和的重构后的程度。

Tags

Nucleosomal ArrayRecombinant Core HistonesNucleosome Positioning DNASedimentation VelocityAnalytical UltracentrifugeAtomic Force MicroscopyChromatin StructureChromatin Function

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

从鼠尾基因组DNA提取

结合QD - FRET技术和微流体实时监测DN​​A纳米复合自组装

大会，加载和分析超速离心机样品池对齐

超高分子量基因组DNA的提取土壤和沉积物

从基因组DNA选择性捕获的5 - 羟甲基

复用的恒化器阵列的设计与应用

高效的生产和纯化重组小鼠Kindlin-3从昆虫细胞生物物理研究

单工程的RNA转录物的活细胞实时成像采用比例式双分子信标

重组弹性蛋白样多肽及其融合物与从肽和蛋白质非色谱纯化<em>大肠杆菌</em

逆转录病毒整合位点的扩增，新一代测序和基因组DNA制图

Read Article