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Chapters
- 00:05Title
- 01:18Excising the Embryonic Hearts
- 02:59Culture Set-up
- 04:18Fixation and Visualization
- 05:09Representative Embryonic Heart Culturing Results
- 07:27Conclusion
Developmental studies in the mouse are hampered by the inaccessibility of the embryo during gestation. To promote the long-term culture of the embryonic heart at late stages of gestation, we developed a protocol in which the excised heart is cultured in a semi-solid, dilute Matrigel.
