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Biology

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Isolation of Embryonic Ventricular Endothelial Cells
 

Isolation of Embryonic Ventricular Endothelial Cells

Article DOI: 10.3791/50463-v 08:05 min July 20th, 2013
July 20th, 2013

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Summary

Primary cell culture is a useful technique for analyzing specific populations of cells, particularly from transgenic mouse embryos at specific developmental stages. Herein, embryonic ventricles are dissected and dissociated, and antibody-conjugated beads recognize and separate out the endothelial cells for further analysis.

Tags

Keywords: Embryonic Ventricular Endothelial Cells Cell Culture Primary Cells Transgenic Mouse Magnetic Beads Cell Isolation Trypsin Developing Coronary Vasculature Embryonic Stages
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