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Oral Transmission of Listeria monocytogenes in Mice via Ingestion of Contaminated Food
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Immunology and Infection
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JoVE Journal Immunology and Infection
Oral Transmission of Listeria monocytogenes in Mice via Ingestion of Contaminated Food

Oral Transmission of Listeria monocytogenes in Mice via Ingestion of Contaminated Food

DOI:
10.3791/50381-v

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08:38 min

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May 06, 2013

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, , ,
1Department of Microbiology, Immunology, and Molecular Genetics,University of Kentucky

Chapters

  • 00:05Title
  • 01:51Preparation of the Inoculum
  • 02:52Infection of Mice
  • 03:50Monitoring the Level of Bacteria Shed in Feces
  • 04:36Harvest of Infected Tissues
  • 05:58Processing of Infected Intestinal Tissues
  • 06:59Results: Food Borne Infection
  • 08:00Conclusion

Summary

Automatic Translation

Automatic Translation

This paper describes a novel method for oral infection of mice using Listeria monocytogenes-contaminated food. The protocol can readily be adapted for use with other food borne bacterial pathogens.

Tags

Key Keywords: Listeria MonocytogenesOral TransmissionFood-borne InfectionMouse ModelGastrointestinal InfectionSystemic SpreadBALB/c/By/JC57BL/6Gall BladderBrainVirulenceHost Response

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