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Chapters
- 00:05Title
- 02:53HeLa Cell Culture on Carbon-coated, Gold EM Finder Grids
- 03:54HIV-1 Infection and Live-cell Imaging
- 05:19Frozen-hydrated EM Sample Preparation
- 06:14Cryo-fluorescence Light Microscopy
- 07:51Cryo-electron Tomography
- 08:56Three-dimensional Reconstruction using IMOD
- 09:33Results: Observing Dynamic Behavior of Small HIV-1 Particles Interacting with Host HeLa Cells
- 12:45Conclusion
Vi beskriver ett korrelat mikroskopi metod som kombinerar hög hastighet 3D live-cell fluorescerande ljus mikroskopi och högupplösta Cryo-elektron tomografi. Vi visar förmågan hos korrelativa metod genom avbildning dynamisk, små HIV-1 partiklar interagerar med värd-HeLa-celler.
