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Accelerated diabete di tipo 1 nei topi induzione trasferimento adottivo di cellule CD4 + T Cells diabetogenici
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells
DOI:

06:27 min

May 06, 2013

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Chapters

  • 00:05Title
  • 01:11Isolation of T Cells from Spleen and Lymph Nodes of BDC2.5 Mice
  • 02:52Fluorescence-activated Cell Sorting of Diabetogenic CD4+ T Cells from BDC2.5 Mice
  • 04:28Adoptive Transfer of Diabetogenic CD4+ T Cells from BDC2.5 Mice
  • 05:12Monitoring Recipient Mice for Hyperglycemia and T1D
  • 05:36Results: Adoptive T-cell Transfer
  • 06:10Conclusion

Summary

Automatic Translation

Noi forniamo un metodo riproducibile per indurre il diabete di tipo 1 (T1D) nei topi entro due settimane dal trasferimento adottivo di isolotto antigene-specifiche, CD4 + cellule T primarie.

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