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应用的<em在体外</em>保护DNA检测,以可视化应力调解属性的DPS蛋白质
JoVE Journal
Biology
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JoVE Journal Biology
Application of an In vitro DNA Protection Assay to Visualize Stress Mediation Properties of the Dps Protein
DOI:

12:41 min

May 31, 2013

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Chapters

  • 00:05Title
  • 01:43Dps Expression and Purification
  • 06:46DNA Protection Assay
  • 09:42Results: Purified Dps Protects DNA from Oxidative Degradation
  • 11:58Conclusion

Summary

Automatic Translation

(DPS)从饥饿细胞的DNA结合蛋白起着至关重要的作用打击细菌应力的。本文讨论了净化<em> E。大肠杆菌</em> DPS和一个协议<em>在体外</em>试验示范DPS-介导的保护DNA降解的活性氧物种。

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