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Chapters
- 00:05Title
- 02:40Amplification of the Template
- 04:01Vacuum Workstation Protocol
- 04:05Immobilizing the Biotinylated PCR Products
- 05:06Denaturation of DNA and Addition of Sequencing Primer
- 07:30Annealing Sequencing Primer to DNA Strands
- 07:55Pyromark Q24 Operation for Pyrosequencing
- 07:58Preparing for a Run
- 08:58Starting a Run
- 09:46Completing a Run
- 10:03Results: Pyrosequencing Identifies Bacteria and Detects Antibiotic Drug Resistance
- 11:35Conclusion
Pyrosequencing er en allsidig teknikk som muliggjør mikrobiell sekvenserings som kan brukes til å identifisere bakteriearter, diskriminere bakteriestammer, og detektere genetiske mutasjoner som gir resistens til anti-mikrobielle midler. I denne video, vil prosedyren for mikrobiell generering amplikon, amplikon pyrosequencing, og DNA-sekvens-analyse påvises.
Tags
Keywords:
PyrosequencingMicrobial IdentificationMicrobial CharacterizationBacterial Species IdentificationBacterial Strain DiscriminationAntimicrobial Resistance DetectionHigh-throughput ScreeningMicrobial Genome SequencingSingle Base SequencingChemiluminescent DetectionReal-time SequencingSNP TypingMutation Detection
