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Scalable, Flexible, and Cost-Effective Seedling Grafting
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JoVE Journal Biology
Scalable, Flexible, and Cost-Effective Seedling Grafting

Scalable, Flexible, and Cost-Effective Seedling Grafting

DOI:
10.3791/64519-v

•

09:33 min

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January 06, 2023

•

,
1Department of Plant and Microbial Biology,North Carolina State University, 2Plants for Human Health Institute,North Carolina State University

Chapters

  • 00:04Introduction
  • 00:20Preparation of the Silicone Grafting Device
  • 02:01Preparation of Seedlings for Grafting
  • 04:39Procedure for Grafting
  • 07:25Results: Grafting Trial Variables and Success Rates
  • 09:06Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a robust seedling grafting method that requires no prior experience or training and can be executed at a very low cost using materials easily accessible in most molecular biology labs.

Tags

Seedling GraftingSeeding GraftingSilicone ElastomerWirePetri DishSilicon SheetAutoclaveSeed SterilizationArabidopsisVernalizationMS Plate

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