/

/

MBP基于标记策略的Mgm101重组蛋白的制备

JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy

Please note that all translations are automatically generated. Click here for the English version.

MBP基于标记策略的Mgm101重组蛋白的制备

DOI:

10.3791/50448-v

•

11:40 min

•

June 25, 2013

•

Xiaowen Wang, MacMillan Mbantenkhu, Sara Wierzbicki, Xin Jie Chen

¹Department of Biochemistry and Molecular Biology,State University of New York Upstate Medical University

Chapters

00:05Title
02:10Construction of MBP-Mgm101 Fusion Protein
03:16Expression, Induction, Cell Lysis and DNase I Treatment
05:28Purification on Amylose Column
07:07PreScission Protease Cleavage and Cation Exchange Chromatography
08:42Size Exclusion Chromatography
10:27Analysis of Purified Mgm101
11:23Conclusion

Summary

Automatic Translation

酵母线粒体类核蛋白质，Mgm101，是一种重组蛋白RAD52型，形成大低聚环。甲协议的描述以制备可溶性的重组Mgm101使用麦芽糖结合蛋白（MBP）标记加上阳离子交换和尺寸排阻色谱法策略。

Tags

Keywords: Mgm101 Recombinant Protein MBP-tagged E. Coli Expression Affinity Chromatography Cationic Exchange Chromatography Size Exclusion Chromatography Mitochondrial DNA Repair Rad52-type Recombination Proteins

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

利用数学建模预测的人口更替策略的有效性

无有机溶剂和醋酸，蛋白质与考马斯亮蓝G - 250凝胶染色

液相恢复GELFREE 8100分馏系统使用基于分子量分馏

使用内源性野生型BRCA1基因的细胞表达，确定同源重组的BRCA1基因突变的影响

利用同位素标记的质谱剖析巯基氧化还原蛋白质

基因改造和重组唾液的腺体器官培养

提高蛋白质结晶的成功率随机Microseed矩阵筛选

分段微管的制备研究运动驱动的微管拆解完

膜蛋白的绿色荧光蛋白为基础的表达筛选<em>大肠杆菌</em

秀丽线虫绿色荧光蛋白标记和 4 '、6-Diamidino-2-苯基吲哚染色检测蛋白质亚细胞定位

Read Article