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Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture
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JoVE Journal Neuroscience
Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture

Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture

DOI:
10.3791/50487-v

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12:38 min

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September 16, 2013

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, ,
1Section of Endocrinology,Denver VA Medical Center, 2Department of Medicine,University of Colorado Denver School of Medicine

Chapters

  • 00:00Title
  • 01:51Expansion and Differentiation of Human Neuroprogenitor Cells
  • 05:28Immunofluorescence Staining of Differentiated Neuroprogenitor
  • 10:17Results: Laser Capture Microdissection of Differrntiated Neurons
  • 12:14Conclusion

Summary

Automatic Translation

Automatic Translation

Human neuroprogenitor cells (NPCs) were expanded under proliferating conditions. NPCs were differentiated into neuron-rich cultures in the presence of a combination of neurotrophins. Neuronal markers were detected by immunofluorescence staining. To isolate a pure population of neurons, NPCs were differentiated on PEN membrane slides and laser capture microdissection was performed.

Tags

Laser Capture MicrodissectionNeuronsDifferentiated Human Neuroprogenitor CellsCell CultureNeuronal MarkersAstrocyte MarkersNeuNSynapsinAcetylcholinesteraseSynaptophysinGAP43GFAPSTAT3Molecular Characterization

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