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Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
 

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Article DOI: 10.3791/50488-v • 10:16 min • February 8th, 2014
February 8th, 2014 •
1,2, 1, 1
1The Rowland Institute, Harvard University, 2Faculdade de Ciências e Letras de Assis, Universidade Estadual Paulista

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Summary
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The three-dimensional locations of weakly-scattering objects can be uniquely identified using digital inline holographic microscopy (DIHM), which involves a minor modification to a standard microscope. Our software uses a simple imaging heuristic coupled with Rayleigh-Sommerfeld back-propagation to yield the three-dimensional position and geometry of a microscopic phase object.

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Keywords: Digital Inline Holographic Microscopy Weakly-scattering Objects Phase Contrast DIC 3D Reconstruction Rayleigh-Sommerfeld Back Propagation Gouy Phase Anomaly CMOS CCD LED Laser Illumination
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