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胰蛋白酶精华协议分析视网膜血管小鼠模型
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Neuroscience
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JoVE Journal Neuroscience
Trypsin Digest Protocol to Analyze the Retinal Vasculature of a Mouse Model
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胰蛋白酶精华协议分析视网膜血管小鼠模型

DOI:
10.3791/50489-v

•

09:03 min

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June 13, 2013

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, ,
1Department of Ophthalmology,Northwestern University Feinberg School of Medicine

Chapters

  • 00:05Title
  • 01:34Retinal Preparation
  • 02:22Water Washes and Trypsin Digest
  • 03:33Separating the Vasculature
  • 05:32Flat-mounting the Vasculature
  • 06:13Results: Representative Images of Trypsin Digest
  • 08:07Conclusion

Summary

Automatic Translation

Automatic Translation

胰蛋白酶的消化是一个最常用的方法来分析视网膜血管。这份手稿中详细介绍的方法，包括关键的改动，优化技术，并删除无血管组织，同时保持整体架构的船只。

Tags

Retinal VasculatureTrypsin DigestMouse ModelFlat-mount Retinal Blood VesselsRetinal Vascular ChangesMicroaneurysmsCapillary DegenerationEndothelial To Pericyte RatiosMouse EyeRetinal Blood VesselsRetinal Blood Vessel Architecture

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