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Chapters
- 00:05Title
- 01:17Mosaic Fly Generation
- 02:17Heat Fixation of Selected Larvae
- 04:02In vivo Imaging of Tracheal Terminal Cells
- 06:08Analysis and Quantification of Terminal Cell Morphology Using ImageJ
- 07:19MARCM-labeled Trachael Cells
- 08:00Conclusion
Here, we present a method for light microscopy analysis of tracheal terminal cells in Drosophila larvae. This method allows for quick examination of branch and lumen morphology in whole animals and would be useful for analysis of individual mutants or screens for mutations affecting terminal cell development.
