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JoVE Journal
Biology

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Visualization of Craniofacial Development in the sox10
 

Visualization of Craniofacial Development in the sox10: kaede Transgenic Zebrafish Line Using Time-lapse Confocal Microscopy

Article DOI: 10.3791/50525-v 06:35 min September 30th, 2013
September 30th, 2013

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Summary

Visualization of experimental data has become a key element in presenting results to the scientific community. Generation of live time-lapse recording of growing embryos contributes to better presentation and understanding of complex developmental processes. This protocol is a step-by-step guide to cell labeling via photoconversion of kaede protein in zebrafish.

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Keywords: Craniofacial Development Sox10: Kaede Transgenic Zebrafish Time-lapse Confocal Microscopy Vertebrate Palatogenesis Cranial Neural Crest Cells Lineage Analysis Maxillary Mandibular Ethmoid Plate Photoconvertible Reporter Protein Zebrafish Embryo
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