JoVE Journal
Biology
Biology
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Summary
Please note that all translations are automatically generated.
可广泛用于微型热泳(MST)无需纯化从细胞裂解液中的目标蛋白的结合亲和力的测定。该协议涉及过表达GFP融合蛋白,在非变性条件下的细胞裂解,MST信号检测在不同浓度的配体的存在下。