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一种多功能自动化平台微尺度细胞刺激实验

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

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一种多功能自动化平台微尺度细胞刺激实验

DOI:

10.3791/50597-v

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12:21 min

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August 06, 2013

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Anupama Sinha, Mais J. Jebrail, Hanyoup Kim³, Kamlesh D. Patel, Steven S. Branda

¹Department of Systems Biology,Sandia National Laboratories, ²Department of Biotechnology and Bioengineering,Sandia National Laboratories, ³Canon U.S. Life Sciences, ⁴Department of Advanced Systems Engineering and Deployment,Sandia National Laboratories

Chapters

00:05Title
01:59Assembly and Population of the Cell Perfusion Chambers
04:28Fabrication of the Digital Microfluidic (DMF) Module and Assembly of the Hub Architecture
06:37Stimulation of Cell Populations
07:45Terminate Stimulation and Recovery of Cell Lysates
09:19Results: Micro-scale Cell Stimulation Produces Results Similar to Conventional Cultures
11:16Conclusion

Summary

Automatic Translation

微观尺度细胞刺激实验，我们已经开发了一个自动细胞培养和审讯平台。该平台提供简单，功能多样，培育和刺激小的细胞群体，并恢复裂解分子分析和精确的控制。该平台非常适合使用珍贵的细胞和/或试剂的研究。

Tags

Automated Platform Micro-scale Cell Stimulation In Vitro Analysis Micro-scale Cultures Digital Microfluidics Cell Perfusion Chambers Cell Culture Immune Cell Response Transcriptional Responses Reagent Consumption Experiment Variability

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