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En alsidig Automatiseret platform for Micro-skala cellestimulering Eksperimenter
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
DOI:

12:21 min

August 06, 2013

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Chapters

  • 00:05Title
  • 01:59Assembly and Population of the Cell Perfusion Chambers
  • 04:28Fabrication of the Digital Microfluidic (DMF) Module and Assembly of the Hub Architecture
  • 06:37Stimulation of Cell Populations
  • 07:45Terminate Stimulation and Recovery of Cell Lysates
  • 09:19Results: Micro-scale Cell Stimulation Produces Results Similar to Conventional Cultures
  • 11:16Conclusion

Summary

Automatic Translation

Vi har udviklet en automatiseret cellekultur og forhør platform for mikro-skala cellestimulering eksperimenter. Platformen tilbyder enkel, alsidig og præcis kontrol i at dyrke og stimulere små populationer af celler, og inddrive lysater til molekylær analyser. Platformen er velegnet til undersøgelser, der anvender værdifulde celler og / eller reagenser.

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