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Neuroscience
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偏振的实验性脑损伤小胶质细胞/巨噬细胞标志物的三维共聚焦分析
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury
Please note that all translations are automatically generated.
Click here for the English version.
偏振的实验性脑损伤小胶质细胞/巨噬细胞标志物的三维共聚焦分析
DOI:
10.3791/50605-v
•
13:28 min
•
September 04, 2013
•
Carlo Perego*
1
,
Stefano Fumagalli*
1
,
Maria-Grazia De Simoni
1
Department of Neuroscience
,
IRCCS – Istituto di Ricerche Farmacologiche Mario Negri
Chapters
00:05
Title
01:45
Immunofluorescence
04:52
Acquisition of Three-dimensional Images by Confocal Microscopy
07:42
Three-dimensional View of Confocal Acquisitions and Three-dimensional Rendering
10:12
Representative Imaging Results
12:46
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
一种方式来获得新的见解的大脑炎症反应的复杂性呈现。我们描述了免疫为基础的协议,其次是三维共聚焦分析,以探讨局灶性脑缺血小鼠模型共表达小胶质细胞/巨噬细胞表型标志物的图案。
Tags
3D Confocal Analysis
Microglia
Macrophage
Polarization
Brain Injury
Ischemia
M1
M2
CD11b
CD68
Ym1
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