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Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury
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Chapters
- 00:05Title
- 01:45Immunofluorescence
- 04:52Acquisition of Three-dimensional Images by Confocal Microscopy
- 07:42Three-dimensional View of Confocal Acquisitions and Three-dimensional Rendering
- 10:12Representative Imaging Results
- 12:46Conclusion
A way to gain new insights into the complexity of the brain inflammatory response is presented. We describe immunofluorescence-based protocols followed by three-dimensional confocal analysis to investigate the pattern of co-expression of microglia/macrophage phenotype markers in a mouse model of focal ischemia.
