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Chapters
- 00:05Title
- 02:00Large Scale Lipid A Extraction (50 ml to 1.5 L)
- 06:22Electrospray Ionization Mass Spectrometry of Lipid A and Collision Induced Dissociation
- 07:56MS/MS on Lipid A by Ultraviolet Photodissociation (UVPD)
- 09:04Results: Chemical Characterization of Lipid A by Mass Spectrometry and Thin Layer Chromatography
- 12:00Conclusion
Isolation and characterization of the lipid A domain of lipopolysaccharide (LPS) from gram-negative bacteria provides insight into cell surface based mechanisms of antibiotic resistance, bacterial survival and fitness, and how chemically diverse lipid A molecular species differentially modulate host innate immune responses.
