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脂肪酸组分和含量微藻分析
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JoVE Journal Environment
Analysis of Fatty Acid Content and Composition in Microalgae
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脂肪酸组分和含量微藻分析

DOI:
10.3791/50628-v

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07:44 min

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October 01, 2013

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, , , , , ,
1Bioprocess Engineering,Wageningen University and Research Center, 2AlgaePARC,Wageningen University and Research Center, 3Food and Biobased Research,Wageningen University and Research Center

Chapters

  • 00:05Title
  • 00:57Sample Preparation
  • 02:03Cell Disruption and Lipid Extraction
  • 03:44Transesterification to fatty Acid Methyl Esters
  • 04:51Quantification of Fatty Acid Methyl Esters Using Gas Chromatography
  • 05:25Results: Fatty Acid Content and Composition of Microalgae as Determined by Gas Chromatography
  • 07:19Conclusion

Summary

Automatic Translation

Automatic Translation

一种用于脂肪酸含量和组成在微藻基于机械破碎细胞，溶剂为基础的脂质提取，酯交换，以及量化和鉴定用气相色谱法的脂肪酸的测定方法进行说明。一个tripentadecanoin内标是用来弥补提取和不完整的酯交换过程中可能发生的损失。

Tags

Fatty Acid ContentFatty Acid CompositionMicroalgaeLipid ExtractionTransesterificationGas ChromatographyTAG Internal StandardFAME Analysis

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