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Journal / Chemistry / Matrix-assisted Laser Desorption/Ionization Time…
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Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
 

Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa

Article DOI: 10.3791/50635-v • 07:49 min • September 9th, 2013
September 9th, 2013 •
1, 1
1Institute of Structural Biology "J.P. Ebel", UMR5075, Commissariat à L'Energie Atomique et aux Energies Alternatives (CEA), Centre National de la Recherche Scientifique (CNRS), Université J. Fourier

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Summary
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Accurate mass measurement represents an important step during the investigation of proteins. Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) can be used for such determination and its key advantage is the tolerance to salts, detergents and contaminants. Here we illustrate an accessible approach for the analysis of proteins larger than 100 kDa by MALDI-MS.

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Keywords: MALDI-TOF Mass Spectrometry Intact Proteins Protein Analysis Protein Characterization Large Proteins Protein Mass Determination Sample Preparation Matrix Calibration Laser Energy Acquisition Time
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