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呼吸爆发的响应为先天免疫健康的斑马鱼中的指标量化

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Immunology and Infection

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JoVE Journal Immunology and Infection

Quantification of the Respiratory Burst Response as an Indicator of Innate Immune Health in Zebrafish

Please note that all translations are automatically generated. Click here for the English version.

呼吸爆发的响应为先天免疫健康的斑马鱼中的指标量化

DOI:

10.3791/50667-v

•

07:07 min

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September 12, 2013

•

Michelle F. Goody, Eric Peterman, Con Sullivan, Carol H. Kim

¹Department of Molecular and Biomedical Sciences,University of Maine

Chapters

00:05Title
01:02Zebrafish Care and Maintenance
01:59Microplate Reader Programming
03:57Fluorescence Quantification
04:34Data Analysis
05:41Results: Respiratory Burst Response in Zebrafish Embryos
06:41Conclusion

Summary

Automatic Translation

先天免疫反应保护免受病原体感染的生物体。先天免疫反应的一个重要组成部分，吞噬细胞呼吸爆发，产生活性氧杀死入侵的微生物。我们描述了量化时所产生的先天免疫反应的化学诱导的活性氧呼吸爆发测定。

Tags

Respiratory Burst Innate Immunity Zebrafish Reactive Oxygen Species Phagocyte Fluorometric Assay Microplate Reader Fluorogenic Substrate Developmental Stage Kidney Fish Species

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