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的代谢活性细菌在通才的肠道鉴定<em>海灰翅夜蛾</em>通过DNA的稳定同位素探测使用<sup> 13</sup> C-葡萄糖
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Identification of Metabolically Active Bacteria in the Gut of the Generalist Spodoptera littoralis via DNA Stable Isotope Probing Using 13C-Glucose
Please note that all translations are automatically generated. Click here for the English version.

的代谢活性细菌在通才的肠道鉴定<em>海灰翅夜蛾</em>通过DNA的稳定同位素探测使用<sup> 13</sup> C-葡萄糖

DOI:
10.3791/50734-v

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12:11 min

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November 13, 2013

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, ,
1Department of Bioorganic Chemistry,Max Planck Institute for Chemical Ecology

Chapters

  • 00:05Title
  • 02:3513C-Labeling of DNA in Metabolically Active Gut Bacteria
  • 03:42Insect Dissection
  • 05:06DNA Extraction and Amplification
  • 06:13CsCl Gradient/DNA Ultracentrifugation
  • 08:27Processing the CsCl Gradients
  • 09:45Identifying Metabolically Active Gut Bacteria
  • 11:13Conclusion

Summary

Automatic Translation

Automatic Translation

与海灰翅夜蛾的肠道相关活动的细菌群落，由稳定同位素探测（SIP）耦合到焦磷酸测序确定。使用这种方法，识别社区内的代谢活性细菌种类与高的分辨率和精度完成。

Tags

Stable Isotope Probing13C-glucoseMetabolically Active BacteriaSpodoptera LittoralisInsect GutMicrobial CommunityPyrosequencingCulture-independent TechniquesDNA Density Gradient Centrifugation

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