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Isolation of Microvascular Endothelial Tubes from Mouse Resistance Arteries
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Biology
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JoVE Journal Biology
Isolation of Microvascular Endothelial Tubes from Mouse Resistance Arteries

Isolation of Microvascular Endothelial Tubes from Mouse Resistance Arteries

DOI:
10.3791/50759-v

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09:23 min

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November 25, 2013

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1Medical Pharmacology and Physiology,University of Missouri, 2Dalton Cardiovascular Research Center

Chapters

  • 00:05Title
  • 01:28Isolation of the Superior Epigastric Artery (SEA)
  • 04:24Isolation of the Endothelial Tube
  • 08:08Results: Representative Calcium Fluorescence in an Endothelial Cell Tube
  • 08:46Conclusion

Summary

Automatic Translation

Automatic Translation

We present a preparation for visualizing and manipulating calcium signaling in native, intact microvascular endothelium. Endothelial tubes freshly isolated from mouse resistance arteries supplying skeletal muscle retain in vivo morphology and dynamic signaling within and between neighboring cells. Endothelial tubes can be prepared from microvessels of other tissues and organs.

Tags

Keywords: Microvascular Endothelial TubesResistance ArteriesEndothelial CellsBlood Flow ControlCa2+ SignalingVasodilationNitric OxideArachidonic AcidSuperior Epigastric ArteryEnzymatic DigestionGap JunctionsCalcium SignalingMembrane BiophysicsGene ExpressionProtein Localization

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