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隔离和幼稚的CD4 T细胞的Th17细胞分化
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
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隔离和幼稚的CD4 T细胞的Th17细胞分化

DOI:
10.3791/50765-v

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12:59 min

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September 26, 2013

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, , , ,
1Department of Microbiology and Cell Science,The University of Florida

Chapters

  • 00:05Title
  • 01:27Plate-bound anti-CD3
  • 02:03Mouse Dissection
  • 04:40Th17 Differentiation
  • 05:40qPCR and ELISA
  • 06:27Cell Activation
  • 07:09Intracellular Staining
  • 08:55Flow Cytometric Analysis
  • 09:24Results: Isolation and Differentiation of Th17 Cells
  • 12:14Conclusion

Summary

Automatic Translation

Automatic Translation

与效应器功能从其它的T细胞亚群的不同的，Th17细胞已被集中牵连的炎性自身免疫性疾病。这种体外 Th17细胞分化协议提供了一种方法来确定初始CD4 + T淋巴细胞是否能够分化成Th17细胞，并进一步研究其在自身免疫和宿主反应的作用。

Tags

Keywords: Th17 CellsCD4+ T LymphocytesAutoimmune DisordersIL-17T Cell DifferentiationFlow CytometryQPCRELISA

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