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JoVE Journal
Bioengineering

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Procedure for the Development of Multi-depth Circular Cross-sectional Endothelialized Microchannels-on-a-chip
 

Procedure for the Development of Multi-depth Circular Cross-sectional Endothelialized Microchannels-on-a-chip

Article DOI: 10.3791/50771-v 10:55 min October 21st, 2013
October 21st, 2013

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Summary

A microchannels-on-a-chip platform was developed by the combination of photolithographic reflowable photoresist technique, soft lithography, and microfluidics. The endothelialized microchannels platform mimics the three-dimensional (3D) geometry of in vivo microvessels, runs under controlled continuous perfusion flow, allows for high-quality and real-time imaging and can be applied for microvascular research.

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Keywords: Multi-depth Circular Cross-sectional Microchannels Endothelialized Microchannels In Vitro Microvessels 3D Geometry Continuous Fluid Flow Photolithographic Reflow Soft Lithography Microfluidics PDMS Primary Human Umbilical Vein Endothelial Cells (HUVECs) Perfusion
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