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芯片上的压力诱导抗生素易感性测试
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Bioengineering
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JoVE Journal Bioengineering
Stress-induced Antibiotic Susceptibility Testing on a Chip
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芯片上的压力诱导抗生素易感性测试

DOI:
10.3791/50828-v

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12:41 min

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January 08, 2014

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, , , ,
1Fraunhofer USA Center for Manufacturing Innovation, 2Division of Infectious Diseases, Department of Medicine, Brigham and Women’s Hospital,Harvard Medical School, 3Department of Mechanical Engineering,Boston University, 4Department of Biomedical Engineering,Boston University

Chapters

  • 00:05Title
  • 01:40Making the Poly(dimethylsiloxane) (PDMS) Layer
  • 02:29Assembling the Flow Cell
  • 03:41Loading the Bacterial Suspension
  • 05:08Loading the Solutions
  • 06:15Image Acquisition
  • 08:02Disinfecting the Flow Cell and Counting the Bacteria
  • 09:21Results: Representative Bacterial Susceptibility Imaging and Survival Data
  • 12:07Conclusion

Summary

Automatic Translation

Automatic Translation

我们开发了一个用于快速抗生素易感性检测的微流体平台。流体以高速通过在微流体通道底部固定的细菌。在压力和抗生素的存在下，易感菌株会迅速死亡。然而，耐药细菌可以在这些紧张的条件下存活下来。

Tags

MicrofluidicsAntibiotic Susceptibility TestingStress-based TestingBacterial CulturePDMSCell ViabilityFluorescenceImage ProcessingMechanical StressEnzymesMicroarray SubstratesDrug DevelopmentBacterial Suspension Experiments

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