A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Microbiology,Icahn School of Medicine at Mount Sinai, 2Global Health and Emerging Pathogens Institute,Icahn School of Medicine at Mount Sinai, 3Department of Medicine,Icahn School of Medicine at Mount Sinai, 4Department of Microbiology and Immunology, School of Medicine and Dentistry,University of Rochester
Chapters
- 00:05Title
- 01:37Infection of Mammalian Cells with the Recombinant Modified Vaccinia Ankara Virus Expressing the Bacteriophage T7 RNA Polymerase (MVA-T7)
- 02:40Transfection of Mammalian Cells
- 03:48Co-culture of Mammalian Cells with Avian Cells
- 05:22Infection of Chicken Embryonated Eggs
- 06:31Harvest of Allentoic Fluid from Infected Chicken Embryonated Eggs
- 07:52Hemagglutination (HA) Assay
- 09:03Results: Transfection and MVA-T7 Infection Efficiencies and Hemagglutination Assay
- 10:33Conclusion
Newcastle Disease Virus (NDV) wurde ausgiebig in den letzten Jahren, um neue Vektoren für Impfung und Therapie zu entwickeln, unter anderem untersucht. Diese Studien wurden durch Techniken, um rekombinante Virus aus cDNA zu retten, wie die, die wir hier beschreiben, möglich.
