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BMP-2的共价结合利用表面上的自组装单层膜法
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach
DOI:

10:23 min

August 26, 2013

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Chapters

  • 00:05Title
  • 01:28Preparation of Homogeneous Gold Layers and Surface Immobilization of Bone Morphogenetic Protein 2
  • 03:51Surface Characterization
  • 04:45Analysis of Biological Activity
  • 06:25Results: Cellular Response to Surface-immobilized rhBMP-2
  • 09:53Conclusion

Summary

Automatic Translation

我们描述了用于实现BMP-2的有效固定化在表面上的方法。我们的方法是基于一个自组装单层实现共价通过其游离胺残基的BMP-2的结合的形成。这个方法是研究信令在细胞膜的有用工具。

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