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Chemistry
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BMP-2的共价结合利用表面上的自组装单层膜法
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Chemistry
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JoVE Journal
Chemistry
Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach
Please note that all translations are automatically generated.
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BMP-2的共价结合利用表面上的自组装单层膜法
DOI:
10.3791/50842-v
•
10:23 min
•
August 26, 2013
•
Theresa L. M. Pohl
,
Elisabeth H. Schwab
,
Elisabetta A. Cavalcanti-Adam
2
1
Department of Biophysical Chemistry, Institute for Physical Chemistry
,
University of Heidelberg
,
2
Max Planck Institute for Intelligent Systems at Stuttgart
Chapters
00:05
Title
01:28
Preparation of Homogeneous Gold Layers and Surface Immobilization of Bone Morphogenetic Protein 2
03:51
Surface Characterization
04:45
Analysis of Biological Activity
06:25
Results: Cellular Response to Surface-immobilized rhBMP-2
09:53
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了用于实现BMP-2的有效固定化在表面上的方法。我们的方法是基于一个自组装单层实现共价通过其游离胺残基的BMP-2的结合的形成。这个方法是研究信令在细胞膜的有用工具。
Tags
Keywords: BMP-2
Bone Morphogenetic Protein
Self-assembled Monolayer
Covalent Immobilization
Extracellular Matrix
Osteoblast Differentiation
In Vitro Cell Studies
Growth Factor Presentation
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