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Using Coculture to Detect Chemically Mediated Interspecies Interactions
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Using Coculture to Detect Chemically Mediated Interspecies Interactions

Using Coculture to Detect Chemically Mediated Interspecies Interactions

DOI:
10.3791/50863-v

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08:29 min

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October 31, 2013

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1Department of Biology,University of North Carolina at Chapel Hill

Chapters

  • 00:05Title
  • 01:33Prepare and Screen Co-culture Plates
  • 03:20Isolate Putative Inducing Organisms and Streak for Isolated Single Colonies
  • 04:54Retest in Secondary Screen to Confirm Hits
  • 05:54Representative Microbial Interaction Results
  • 07:59Conclusion

Summary

Automatic Translation

Automatic Translation

Bacteria produce secreted compounds that have the potential to affect the physiology of their microbial neighbors. Here we describe a coculture screen that allows detection of such chemically mediated interspecies interactions by mixing soil microbes with fluorescent transcriptional reporter strains of Bacillus subtilis on solid media.

Tags

Keywords: CocultureInterspecies InteractionsChemically Mediated InteractionsFluorescent ReporterMetaboliteBiofilmBacillus SubtilisEnvironmental MicrobesMicrobial CommunitySecondary Screen

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