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肺动脉平滑肌细胞从新生小鼠隔离

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Biology

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JoVE Journal Biology

Isolation of Pulmonary Artery Smooth Muscle Cells from Neonatal Mice

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肺动脉平滑肌细胞从新生小鼠隔离

DOI:

10.3791/50889-v

•

08:02 min

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October 19, 2013

•

Keng Jin Lee, Lyubov Czech, Gregory B. Waypa, Kathryn N. Farrow

¹Department of Pediatrics,Northwestern University Feinberg School of Medicine

Chapters

00:05Title
01:25Pulmonary Artery (PA) Isolation from Neonatal Mice
03:27Culturing Pulmonary Artery Smooth Muscle Cells (PASMC)
06:30Results: Pulmonary Artery Smooth Muscle Cells (PASMC) Expression
07:32Conclusion

Summary

Automatic Translation

我们已经开发了一种新的和可重复的技术来隔离为P7作为年轻的小鼠原代培养的肺动脉平滑肌细胞（PASMC），从而使更好地参与新生儿平滑肌细胞的收缩和舒张的信号转导通路的研究。

Tags

Keywords: Pulmonary Artery Smooth Muscle Cells Pulmonary Hypertension Neonatal Mice Cell Isolation Agarose Iron Particles Collagenase Cell Culture Smooth Muscle Cell Markers

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