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JoVE Journal Chemistry
A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators
 

A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators

Article DOI: 10.3791/50908-v • 10:30 min • December 27th, 2013
December 27th, 2013 •
1, 1, 1, 1, 1,2
1Department of Chemistry, The Scripps Research Institute, 2Worm Institute of Research and Medicine (WIRM), The Scripps Research Institute

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Summary
Automatic Translation

The botulinum neurotoxin type A light chain (BoNT/A LC) is a metalloprotease that enters motor neurons, cleaves its substrate SNAP-25, and disrupts neurotransmission, thereby resulting in flaccid paralysis. Utilizing a high-throughput-compatible FRET-based assay, large libraries of small molecules can be screened for their impact on BoNT/A LC enzymatic activity.

Tags

Botulinum Neurotoxin BoNT Light Chain FRET High-throughput Screening HTS Inhibitor Acetylcholine Neurotransmitter Release Therapeutic Target Small Molecule
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