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JoVE Journal
Immunology and Infection

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Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin
 
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Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin

Article DOI: 10.3791/50952-v 09:10 min December 16th, 2013
December 16th, 2013

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Summary

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Click here for the English version.

Generation of lymph node/fat pad chimeras for the study of lymph node stromal cell origin is described. The method involves the isolation of lymph nodes from newborn mice and embryonic fat pads, the generation of chimeric lymph node-fat pads, and their transfer under the kidney capsule of a host mouse.

Tags

Keywords: Lymph Node Stroma Adipose Tissue Chimera Lineage Tracing Stromal Cell Origin Lymphotoxin-b Receptor Adipocyte Precursor Cells EYFP Flow Cytometry Immunofluorescence
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