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Journal / Immunology and Infection / A CFSE-based Assay to Study the Migration of…
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Immunology and Infection

This content is Open Access.

JoVE Journal Immunology and Infection
A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into Draining Lymph Nodes During Infection with Mycobacterium bovis Bacille Calmette-Guérin
 

A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into Draining Lymph Nodes During Infection with Mycobacterium bovis Bacille Calmette-Guérin

Article DOI: 10.3791/54620-v • 07:14 min • October 9th, 2016
October 9th, 2016 •
1, 1, 1
1Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet

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Summary
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An assay in mice to track cell migration from skin to draining lymph node is described which enables the characterization of skin Dendritic cells mobilized to the lymph node after footpad infection with Bacille Calmette-Guérin.

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Keywords: CFSE Dendritic Cells Migration Draining Lymph Nodes Mycobacterium Bovis BCG Foot Pad Injection Cell Tracking Flow Cytometry
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