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Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas
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Chapters
- 00:05Title
- 01:01Preparing Placenta for Dissection
- 04:49Homogenizing Placental Villous Cells and Isolating Lipid Droplets by Ultracentrifugation
- 07:43Results: Isolation of Lipid Droplets from Placental Villous Cells
- 09:16Conclusion
Two techniques for isolating cellular lipid droplets from 1) yeast cells and 2) human placentas are presented. The centerpiece of both procedures is density gradient centrifugation, where the resulting floating layer containing the droplets can be readily visualized by eye, extracted, and quantified by Western Blot analysis for purity.
