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Bioengineering
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可生物降解聚合物颗粒的淋巴内结注射
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Intra-lymph Node Injection of Biodegradable Polymer Particles
Please note that all translations are automatically generated.
Click here for the English version.
可生物降解聚合物颗粒的淋巴内结注射
DOI:
10.3791/50984-v
•
09:06 min
•
January 02, 2014
•
James I. Andorko*
1
,
Lisa H. Tostanoski*
1
,
Eduardo Solano
,
Maryam Mukhamedova
,
Christopher M. Jewell
1
Fischell Department of Bioengineering
,
University of Maryland, College Park
Chapters
00:05
Title
01:52
Synthesis of Lipid-stabilized Micro and Nanoparticles
03:21
Determination of Particle Size
04:03
Preparation of Mice for
i.LN.
Injection
05:31
i.LN.
Injection of Particles
06:33
Results: Particle Synthesis, Animal Preparation, and Lymph Node Injection
07:19
Results: Tracer Dye Drainage
07:38
Results: Visualization of Particles in Injection Lymph Nodes
08:03
Conclusion
Summary
Automatic Translation
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Automatic Translation
淋巴结是协调免疫反应的免疫组织,是疫苗的关键目标。生物材料已被用于更好地瞄准淋巴结和控制抗原或辅助剂的传递。本文描述了一种将这些想法相结合的技术,将生物相容的聚合物颗粒注入淋巴结。
Tags
Keywords: Lymph Node Injection
Biodegradable Polymer Particles
Adaptive Immune Response
Lymph Node Targeting
Biomaterial Vaccine
Microparticles
Nanoparticles
Double Emulsion
Lipid Stabilizers
Fluorescent Microscopy
Nontoxic Tracer Dye
Biomaterial-based Vaccines
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