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Journal / Neuroscience / Nucleofection of Rodent Neuroblasts to Study…
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Neuroscience

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JoVE Journal Neuroscience
Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro
 

Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro

Article DOI: 10.3791/50989-v • 11:32 min • November 12th, 2013
November 12th, 2013 •
*1, *2, 1, 1, 1
1Wolfson Centre for Age-Related Diseases, King's College London, 2MRC Centre for Developmental Neurobiology, King's College London
* These authors contributed equally

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Summary
Automatic Translation

Neuroblast migration is a crucial step in postnatal neurogenesis. The protocol described here can be used to investigate the role of candidate regulators of neuroblast migration by employing DNA/small hairpin RNA (shRNA) nucleofection and a 3D migration assay with neuroblasts isolated from the rodent postnatal rostral migratory stream.

Tags

Keywords: Subventricular Zone Neuroblast Migration Rostral Migratory Stream Olfactory Bulb Neurogenesis Nucleofection 3D In Vitro Migration Assay Plasmid DNA ShRNA SiRNA Reaggregation Signaling Pathways
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