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皮层小胶质细胞的分离与保存免疫表型和功能从新生儿鼠
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates
DOI:

09:12 min

January 30, 2014

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Chapters

  • 00:05Title
  • 01:19Preparation
  • 02:23Sample Collection
  • 04:31Growing Cortical Cells
  • 05:56Isolation of Microglia from Mixed Glial Culture
  • 07:30Results: Preserved Immunophenotype and Functionality in Isolated Microglia
  • 08:46Conclusion

Summary

Automatic Translation

一键小胶质细胞生物学的研究成功是小胶质细胞免疫功能的体外从中枢神经系统组织分离过程中的保存。通过旋转振动造成的高纯度和immunofunctional细胞培养物中分离的小胶质细胞所评估的荧光成像,免疫细胞化学,ELISA和以下的小胶质细胞活化的促炎性刺激脂多糖(LPS)和Pam 3 CSK 4(PAM)。

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