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Bioengineering
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对于I型胶原蛋白的制备皮肤方法的改进
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
An Improved Method for the Preparation of Type I Collagen From Skin
Please note that all translations are automatically generated.
Click here for the English version.
对于I型胶原蛋白的制备皮肤方法的改进
DOI:
10.3791/51011-v
•
05:17 min
•
January 21, 2014
•
Christina A. Pacak
2
,
Allison A. MacKay
,
Douglas B. Cowan
2
1
Department of Anesthesiology, Perioperative and Pain Medicine
,
Boston Children’s Hospital
,
2
Department of Anesthesia
,
Harvard Medical School
Chapters
00:05
Title
01:10
Preparing the Dermal Sample
01:44
Removing Noncollagenous Solubilized Material
02:48
Type I Collagen Extraction
04:22
Results: Rapid Isolation from Dermis to Yield COL1 Polymerized into Fibrils
04:58
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
对于可溶性Ⅰ型胶原(COL1)隔离的传统程序需要从开始10天左右,由于长时间的缓冲液孵育和纤丝费力再悬浮完成。在这里,我们描述了从真皮小活检净化COL1,在不到3小时的方法。
Tags
Keywords: Type I Collagen
COL1
Collagen Extraction
Skin-derived Collagen
Autologous Collagen
Centrifugal Filtration
Regenerative Applications
Clinical Applications
Cosmetic Surgery
Dermal Injections
Bone Grafting
Reconstructive Surgery
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