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جيل من القص التصاق خريطة طريق SynVivo شبكات الاوعية الدموية الدقيقة الاصطناعية
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Generation of Shear Adhesion Map Using SynVivo Synthetic Microvascular Networks
DOI:

09:52 min

May 25, 2014

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Chapters

  • 00:05Title
  • 02:09Priming the SynVivo-SMN Microfluidic Device
  • 03:01Coating the Microfluidic Device with Desired Protein
  • 03:56Flowing the Biotinylated Particles for Adhesion
  • 05:26Image Acquisition and Particle Flux Analysis Using Computational Fluid Dynamics (CFD) Models
  • 06:50Results: SynVivo-SMN Adhesion Data Analysis
  • 08:44Conclusion

Summary

Automatic Translation

Flow chambers used in adhesion experiments typically consist of linear flow paths and require multiple experiments at different flow rates to generate a shear adhesion map. SynVivo-SMN enables the generation of shear adhesion map using a single experiment utilizing microliter volumes resulting in significant savings in time and consumables.

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