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Chapters
- 00:05Title
- 01:31Setup: The Day Before Tumor Harvest
- 02:21Setup and Tumor Harvest
- 04:08Tissue Dissociation and Trituration
- 07:59Cell Plating
- 09:19Results: Primary Vestibular Schwannoma Cultures
- 10:18Conclusion
Vestibular Schwannomas (VSs) are non-malignant tumors of Schwann cell (SC) origin, associated with mutations in the NF2 tumor suppressor gene. We report a reproducible, efficient protocol for primary human VS cell culture that allows for molecular and cellular experimental manipulation and analysis and recapitulates the heterogeneous nature of human disease.
