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Использование флуоресцентных белков для визуализации и количественной<em> Хламидиоз</em> Вакуоли Динамика роста в живых клетках
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells
DOI:

07:42 min

October 13, 2015

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Chapters

  • 00:05Title
  • 01:21Infecting Cells with Chlamydia
  • 03:04Live Cell Visualization of Chlamydia Inclusions
  • 03:50Quantitation of Inclusion Growth Parameters in Live Cells
  • 05:40Results: Chlamydia Vacuole Growth Dynamics in GFP-HeLa Cells
  • 07:04Conclusion

Summary

Automatic Translation

A live cell fluorescent protein based method for illuminating cellular vacuoles (inclusions) containing Chlamydia is described. This strategy enables rapid, automated determination of Chlamydia infectivity in samples and can be used to quantitatively investigate inclusion growth dynamics.

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