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Immunology and Infection
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Humanized Mouse Model to Study Bacterial Infections Targeting the Microvasculature
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Humanized Mouse Model to Study Bacterial Infections Targeting the Microvasculature

Humanized Mouse Model to Study Bacterial Infections Targeting the Microvasculature

DOI:
10.3791/51134-v

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11:40 min

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April 01, 2014

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, ,
1INSERM U970,Paris Cardiovascular Research Centre, 2Faculté de Médecine Paris Descartes,Université Paris Descartes

Chapters

  • 00:05Title
  • 01:26Skin Graft
  • 03:40Infection
  • 05:41Organ CFU Counts
  • 07:52Results: Representative CFU Counts and Histologies of Infected Human Tissue
  • 11:14Conclusion

Summary

Automatic Translation

Automatic Translation

Neisseria meningitidis is a human specific pathogen that infects blood vessels. In this protocol human microvessels are introduced into a mouse by grafting human skin onto immunocompromised mice. Bacteria adhere extensively to the human vessels, leading to vascular damage and development of the purpuric rash typically observed in human cases.

Tags

Keywords: Humanized Mouse ModelNeisseria MeningitidisBacterial InfectionMicrovascular DamageSepsisVascular LeakPurpuric RashHuman Skin GraftHuman Dermal MicrovesselAnastomosisIn Vivo ModelPathogenesis

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