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Metoder til kvantitativ påvisning af antistof-induceret komplement aktivering på de røde blodlegemer
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Immunology and Infection
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JoVE Journal Immunology and Infection
Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

Metoder til kvantitativ påvisning af antistof-induceret komplement aktivering på de røde blodlegemer

DOI:
10.3791/51161-v

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06:29 min

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January 29, 2014

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, ,
1Department of Immunopathology, Sanquin Research and Landsteiner Laboratory Academic Medical Center,University of Amsterdam, 2Department of Hematology, Academic Medical Center,University of Amsterdam

Chapters

  • 00:05Title
  • 01:21Complement Deposition Analysis and Quantification of Hemolysis
  • 04:34Results: Quantitative Detection of Antibody-induced Complement Activation on RBCs
  • 06:02Conclusion

Summary

Automatic Translation

Automatic Translation

Her beskriver vi to assays til måling af komplement aktivering induceret af antistoffer mod røde blodlegemer. Den største fordel i forhold til de aktuelle analyser er deres kvantitative og let at fortolke naturen.

Tags

AntibodyComplement ActivationRed Blood CellsRBCAlloantibodiesAutoantibodiesAutoimmune Hemolytic AnemiaAIHACoombs TestHemolytic AssayC3 DepositionC4 DepositionFACS AnalysisQuantitative Hemolytic AssaySpectrophotometryHemoglobin

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