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Chapters
- 00:05Title
- 01:45Preparation of the Density Gradient Fraction System and Sucrose Gradients
- 03:04Preparation of Cell Extracts
- 05:10Loading Extracts onto Sucrose Gradients and Ultracentrifugation
- 06:03Fractionation of Cytoplasmic Extracts for Polysome Profiling
- 06:46Extraction of Protein and RNA for Analysis
- 08:45Results: Polysome Profiling Detects Changes in Translation Initiation in Response to Stress Conditions
- 10:01Conclusion
