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Analyse af translationsinitieringskodon under stress betingelser ved Polysomprofiler Profiling
JoVE Journal
Biology
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JoVE Journal Biology
Analysis of Translation Initiation During Stress Conditions by Polysome Profiling
DOI:

10:59 min

May 19, 2014

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Chapters

  • 00:05Title
  • 01:45Preparation of the Density Gradient Fraction System and Sucrose Gradients
  • 03:04Preparation of Cell Extracts
  • 05:10Loading Extracts onto Sucrose Gradients and Ultracentrifugation
  • 06:03Fractionation of Cytoplasmic Extracts for Polysome Profiling
  • 06:46Extraction of Protein and RNA for Analysis
  • 08:45Results: Polysome Profiling Detects Changes in Translation Initiation in Response to Stress Conditions
  • 10:01Conclusion

Summary

Automatic Translation

Her beskriver vi en metode til at analysere ændringer i initiering af mRNA-translation af eukaryote celler som reaktion på stress-tilstande. Denne metode er baseret på hastigheden separation på saccharosegradienter med oversættelse ribosomer fra ikke-translating ribosomer.

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