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Neuroscience
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隔离和肉毒杆菌神经毒素定量从复杂的基质使用BoTest矩阵测定
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Isolation and Quantification of Botulinum Neurotoxin From Complex Matrices Using the BoTest Matrix Assays
Please note that all translations are automatically generated.
Click here for the English version.
隔离和肉毒杆菌神经毒素定量从复杂的基质使用BoTest矩阵测定
DOI:
10.3791/51170-v
•
12:25 min
•
March 03, 2014
•
F. Mark Dunning
,
Timothy M. Piazza
,
Füsûn N. Zeytin
,
Ward C. Tucker
1
BioSentinel Inc., Madison, WI
Chapters
00:05
Title
01:40
Preparation of the Standard Curve
03:33
Prepare Solid Food Unknown Samples
04:56
Plate Setup and BoNT/A Pull Down
06:11
Assay Initiation, Incubation, and Data Collection
07:14
Results: BoTest Matrix Assay
11:43
Conclusion
Summary
Automatic Translation
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Automatic Translation
该BoTest矩阵A型肉毒毒素(肉毒毒素)检测方法快速净化,并从各种样品基质的量化肉毒毒素。在这里,我们提出了一个协议,用于肉毒毒素,从固体和液体基质的检测和定量,并证明有肉毒杆菌,番茄,牛奶的检测。
Tags
Keywords: Botulinum Neurotoxin
BoNT
BoTest Matrix Assays
Immunoprecipitation
Fluorogenic Reporter
Complex Matrices
Pharmaceutical Testing
Food Safety
Rapid Detection
Quantification
Mouse Bioassay
In Vitro Replacement Assays
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