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隔离和肉毒杆菌神经毒素定量从复杂的基质使用BoTest矩阵测定
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Isolation and Quantification of Botulinum Neurotoxin From Complex Matrices Using the BoTest Matrix Assays
DOI:

12:25 min

March 03, 2014

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Chapters

  • 00:05Title
  • 01:40Preparation of the Standard Curve
  • 03:33Prepare Solid Food Unknown Samples
  • 04:56Plate Setup and BoNT/A Pull Down
  • 06:11Assay Initiation, Incubation, and Data Collection
  • 07:14Results: BoTest Matrix Assay
  • 11:43Conclusion

Summary

Automatic Translation

该BoTest矩阵A型肉毒毒素(肉毒毒素)检测方法快速净化,并从各种样品基质的量化肉毒毒素。在这里,我们提出了一个协议,用于肉毒毒素,从固体和液体基质的检测和定量,并证明有肉毒杆菌,番茄,牛奶的检测。

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